Method for evaluation of the fibrous material-cell interaction based on live cell imaging

Tissue engineering is a key area of regenerative medicine, with nano- or microfibrous scaffolds playing a crucial role in promoting cell adhesion, growth and proliferation. For the evaluation of a material’s (or its functionalization) suitability for a given application, it is necessary to have reliable methods for analyzing the behavior of cells on the surface of the material. However, commonly used methods are based on static observation of fixed cells or on determining the biological activity of cells by examining their metabolism. This project aims to develop a new method for evaluating the cell-material interaction, which will be based on live cell imaging on specially developed fibrous scaffolds. This new method will extend the possibilities of material evaluation by a method, which will allow direct observation of the living cells and subsequent statistical processing to evaluate the suitability of the material for a given cell type.